Feature: Mature method for cell proliferation assays, High-throughput.
Cells are structural and functional unit of our body which control and maintain the function of all unicellular and multicellular organisms.
Tracking new RNA synthesis, principle: Detection of newly synthesized RNA is an important method to measure cell proliferation and toxicological profiling.
Determination of cell growth rates is widely used in the testing of drug action and cytotoxic agents, and screening other biologically active compounds.Detecting proliferation markers, principle: Another way to measure cell proliferation is to detect an antigen present in proliferating cells, but not nonproliferating cells, using a monoclonal antibody against the antigen.In the presence of ATP, luciferase produces light (proportional to the ATP concentration) that can photo dvd maker professional for windows 8 be detected by a luminometer or any microplate reader capable of reading luminescent signals.Incubate at 37C for 10 minutes.Among such procedures, the MTT assay developed by Mossman 1 is still one of the most versatile and popular assays.Add 100 L of the SDS-HCl solution (prepared in step.2) to each well and mix thoroughly using the pipette.The age of the cultures, number of passages and details of the growth medium can all be important factors.Our MTT Cell expression web studio 4 ultimate Proliferation Assay Kit provides enough material to perform 1000 individual tests using standard 96-well microplates.Accurate: The data obtained strongly correlates to the cell number.Substrate: 5-ethynyl uridine (EU feature: Simple procedure.
With automated hematology analyzer, the test result of CBC with diff (Complete Blood Count with Differential) can easily be obtained.We are capable of performing different cell proliferation assays based on several concepts, which are measuring rate of DNA replication, analysis of metabolic activity, cell surface antigen recognitions, detecting proliferation markers, ATP Measurement, measures of membrane integrity and.The absorption of the media-containing dye solution can be read using a spectrophotometer or microplate reader in low- or high-throughput configurations.DNA synthesis cell proliferation assays, principle: Proliferating cells incorporate 3H-thymidine/BrdU labels into their nascent DNA, which can be washed, adhered to filters and then measured.Add 10 L of the 12 mM MTT stock solution (prepared in step.1) to each well.In sum, cell proliferation assays are very important in scrutinizing the rate of cell proliferation in both in vitro and in vivo conditions.
Dye Label: 3H-thymidine, BrdU, feature: Traditional method, reliable.